|
KMID : 0350519950480041073
|
|
Journal of Catholic Medical College
1995 Volume.48 No. 4 p.1073 ~ p.1081
|
|
|
Inhibitory Effect of Transforming Growth Factor-β1 on Cellular Proliferation and c-myc Gene Expression in a Chriocarcinoma Cell Line (BeWo)
|
|
|
|
|
Abstract
|
|
|
Transforming growth factorβ1(TGF-β1) is a potent growth inhibitor for many cell types, including most epithelial cells. We studied the effect of TGF-β1 on choriocancinoma cell line in vitro. The effects of TGF-β1 were determned on cell
proliforation
and c-myc mRNA expressio by BeWo cell line. TGF-β1 inhibited the growth of BeWo cells in a dose dependent manner (P=0.02) as measured by MTT ( [3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) assay. When BeWo cells were exposed to
10
ng/ml of TGF-β1, c-myc oncogene expression was suppressed after 4 hr and completely abolished at 24 hr of stimulation by Northern blot analysis. These results show that TGF-β1 may play an antiproliferative effect on BeWo cell growth by
rfeducing
c-myc
gene product. In vitro analysis of cellular and molecular alterations in BeWo cells in this study may aid in elucidating the mechanism of action of TGF-β1 on human choriocarcinoma tissues in vivo.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|